转ER-sHSP基因番茄植株的耐盐性检测

Determination of Salt-tolerance of Transgenic Tomato with ER-sHSP Gene

旨在检测内质网小分子热激蛋白(ER-sHSP)的过量表达对植物耐盐性的影响。利用Hoagland营养液配制200mmol/LNaCl溶液并处理转ER-sHSP基因番茄2周,测定植物的生物量、脯氨酸含量、SOD酶活、MDA含量及相对电导率。结果显示:与对照相比,转基因番茄抗盐表型明显,植物干鲜重分别比对照增加57%和53%,脯氨酸含量和SOD酶活性的平均值分别是对照番茄的1.7倍和3倍,但MDA含量和相对电导率却显著降低(P<0.01)。综上所述,转基因番茄具有较高的耐盐能力,说明ER-sHSP在植物的耐盐机制中发挥重要作用。

To evaluate the physiological function of ER-sHSP, the salt-resistant ability of transgenic tomato plants under salt stress was assessed. Seedlings were treated with Hoagland solution supplemented with 200 mmol/L NaC1 for two weeks followed by determination of biomass, proline content, SOD activity, MDA content and relative electrical leakage. Results showed that transformed lines showed obvious resistance to salt stress compared to the non-transgenic lines. The fresh weight and dry weight of transgenic plants were 57% and 53% higher than that of control lines, respectively. At the same time, the average content of proline and SOD activity of transgenic lines were 1.7 and 3 times higher compared with control lines. However, the MDA content and relative electrical leakage of transgenic lines were significantly lower than the control （P〈0.01）. These results indicated that ER-sHSq played a key role in enhancing the salt-resistance ability of plants.