儿茶素对羟自由基诱发 SOD 基因序列改变的影响

Effects of catechins on spectrum changes of SOD gene induced by hydroxyl radical

利用基因克隆与测序技术 ,研究了儿茶素对羟自由基诱发 SOD基因序列改变的影响 ,结果表明 :Fe( ) /H2 O2 产生的羟自由基可诱导西红柿叶片 SOD基因中 G→ A,G→ C间的颠换 ,且 Fe( )浓度越高 ,颠换率越高 ,尤其是 G→ C的颠换 ,但儿茶素的加入明显抑制这种颠换 ,且 (一 ) - EGCG>(一 ) -ECG>(一 ) - EGC>(一 ) - EC,呈浓度—效应关系 ,而儿茶素本身不能使

By employing the technique of gene cloning and DNA seqencing the effects of hydroxyl radical produced by Fe (Ⅱ) / H 2O 2 reaction were exploring on the spectrum of SOD gene from tomoto leaf and influenced on by catechins. The results showed that the spectrum of Fe (Ⅱ) / H 2O 2 induced changes detected in SOD gene clong assay. The most frequent single base substitution was G→C and G→A. But the ratio of this transition was increased with increasing the concentration of Fe (Ⅱ). It has seriously influence on G→C transition. Moreover, the changes were prohibited after added catechins to the system. The order of prohibition could be classified as follows: (一) EGCG>(一) ECG>(一) EGC>(一) EC. The transitions induced by Fe (Ⅱ) / H 2O 2 were selectively decreased in the presence of catechins and existed the relations between concentration of catechins and effects. Alternatively, catechins by itself could not transit the single base sequence of SOD gene spectrum.