银杏萜类内酯的提取和气相色谱与质谱分析

The extraction and gas chromatographic & mass spectrometric analysis of ginkgolides and bilobalide in \%Ginkgo biloba \%leaves.

该文对银杏内酯A ,B和白果内酯的定性定量分析方法进行研究 .用 2 0 %的甲醇水溶液提取银杏叶中的银杏内酯A ,B和白果内酯 ,用硅胶柱纯化 ,甲苯与丙酮混合液洗脱 ,经硅烷化后质谱定性 ,气相色谱定量 .该方法对白果内酯与银杏内酯A ,B的回收率分别为 93.0 1% ,96 .32 % ,94.6 8% ,相对变异系数分别为 5 75 % ,3 88% ,4 2 3%

A method has been developed for the qualitative and quantitative analysis of ginkgolide A,B and bilobalide in Ginkgo biloba leaves.Components were extracted from \%Ginkgo biloba \%leaves with 20% methanol in water,the extracts were purified by means of silica gel columns with toluene acetone eluation.The trimethylsilylated ginkgolide A,B and bilobalide were determinated by gas chromatography with flame ionization detector and identified by a tetrapolar mass spectrometer detector with autharized chemicals.The recovery of the method for bilobalide and ginkgolide A,B were 93.01%,96.32%,94 68%,RSD 5.75%,3.88%and 4.23% respectively.