RCS大鼠病变过程中视网膜电图振荡电位的频域特性分析

Frequency domain analysis of dark-adapted oscillatory potentials of electroretinogram in RCS rats

目的观察皇家外科学院大鼠(royal college of surgeons,RCS)视网膜变性过程中的暗适应视网膜电图(electroretinogram,ERG)振荡电位(oscillatory potentials,OPs)频域特性。方法选择出生后20、30、40 d及60 d的RCS大鼠各3只,采用RETI-scan记录系统进行闪光视网膜电图(flash electroretinogram,F-ERG)OPs记录,记录电极为环形角膜电极,参考电极为不锈钢针状电极,刺激强度为0dB,通过Matlab提取OPs成分,分析其频谱特性。结果相对于同龄的正常大鼠,RCS大鼠OPs频域幅值明显重度降低(P<0.01),且高频成分缺失。RCS大鼠发育过程中,随着病程进展至40 d时对应的频率向高频部分明显延迟(P<0.05),进展至60 d时其频域幅值明显降低(P<0.05)。结论 RCS大鼠ERG的OPs频域特性与视网膜功能的变性程度存在相关性,利用这些特性可以更明显观察到视网膜变性对大鼠视网膜功能的影响。

Objective To investigate the characteristics of frequency domain of dark-adapted oscilla- tory potentials （ OPs ） of electroretinogram （ERG） in Royal College of Surgeons （ RCS ） rats. Methods Different ages （20, 30, 40 and 60 d） of RCS-rdy--p ＋ rats were involved, and there were three rats of each age. Dark-adapted OPs of flash ERG （F-ERG） was recorded with RETI-scan system. Gold-foil ring cornea electrode was used as the recording electrode and home-made stainless steel needle electrode was used as the reference. The intensity of light was 0 dB. OPs components were extracted by software Matlab 7.0 and the characteristics of OPs were analyzed. Results Compared with the normal rats with same age, the frequency- domain amplitude of RCS rats significantly reduced （P 〈0.01 ） and the high-frequency components of RCS rats disappeared. With the development of the disease, the frequency of the peak amplitude was delayed to high frequency at 40 d （ P 〈 0.05 ）, and the amplitude of the peak reduced significantly at 60 d （ P 〈 0.05 ）. Conclusions There is a correlation between the frequency-domain characteristics of OPs and the development of the disease in RCS rats. By using these characteristics, it is possible to know the influences of the disease on the retinal function of RCS rats.