水稻光温敏核不育基因的分子标记定位研究

Mapping for a Photo-Thermo Sensitive GMS (PTGMS) Gene in Rice

研究了粳型两用不育系D18S和栽培品种黏2杂交的F2群体的主要农艺性状和结实率,并利用水稻第7染色体上的7个SSR标记进行了连锁分析,绘制了遗传图谱,同时利用上述标记对所调查的农艺性状进行了QTL分析。结果表明:①D18S×黏2的F2群体中正常结实株和不育株的比例呈3∶1,说明在D18S/黏2群体的不育性受一个隐性独立基因的控制;②控制D18S的不育性的光敏核不育基因位于第7条染色体长臂段的RM445和RM418之间,其遗传距离分别为4.5cM和0.7cM;③在所标记的区段检测出株高和穗长的QTL,其对株高和穗长的贡献率分别为17.5%和20.0%,另外结实率的QTL能解释83.2%的表型变异。

The F2 population developed from the cross between two Japonica rice cuhivars, ＇D 18S （PT- GMS）＇ and ＇Nian No.2＇, was used to identify quantitative trait loci （QTLs） for major agronomic traits including seed setting rate. The results revealed that the segregation of the normal fertile plant and sterile plant subject to the ratio of 3：1, indicating that the trait PTGMS in the population D18S （PTGMS） × Nian No2 is controlled by a independent recessive gene. A total of seven simple sequence repeat （SSR） markers segregated in the F2 population were distributed over the chromosome 7. The PTGMS gene was mapped between the markers RM445 and RM418 with 4.5 cM and 0.7cM interval. Moreover, two QTLs related with plant height and ear length were also mapped to the same position, which contributes to plant height and ear length by 17.5% and 20.0%. In addition, seed setting rate could interpret 83.2% ofphenotypic variations.