清热润燥口服液止咳、平喘作用及急性毒性实验研究

Experimental Studies on Antitussive and Antiasthmatic Effects and Acute Toxicity of Qingre Runzao Oral Liquid

目的评价清热润燥口服液的止咳、平喘作用及安全性.方法采用小鼠"浓氨水法"及豚鼠"喷雾法"观察清热润燥口服液在止咳、平喘方面的作用;以小鼠最大给药量、最大体积的药量一日1次或2～3次进行灌胃给药,给药后立刻观察动物反应,并连续观察14 d,测小鼠的最大耐受量(MTD).结果清热润燥口服液能延长小鼠咳嗽的潜伏期,减少咳嗽次数(P<0.05);明显能延长豚鼠哮喘的发作潜伏期(P<0.05).急性毒性实验连续观察14 d后,未发生动物死亡,体重均有所增长,观察未见异常反应,小鼠口服给药的最大耐受量是生药量为117.6 g原生药/(kg.d),相当于临床人用量的187倍.结论清热润燥口服液具有止咳和平喘作用.拟用日剂量在临床应用上是安全的.

Objective To observe the antitussive and antiasthmatic effects and the acute toxicity of Qingre Runzao oral liquid. Methods Mice ＂concentrated ammonia method＂ and guinea pig ＂spray method＂ were used to observe the antitussive and antiasthmatic effects of Qingre Runzao oral liquid. The acute toxicity was tested in mice by intragastric administration at the maximum dose and maximum volume, one time or two-three times per day. The animals were observed for 14 days after treatment, and then the maximum tolerated dose （MTD） in mice was determined. Results Qingre Runzao oral liquid could prolong the incubation period of the mice tussive and reduce the frequency of tussive （P 〈 0.05） . It could obviously extend the incubation period of guinea pig asthma attack. There was no death and toxic reaction on mice after treatment for 14 days, and the weight of mice have increased with no abnormal reaction. The MTD in mice by intragastric administration was 117.6 g crude drug （kg.day） , and it was the equivalent of 187 times of the human dose in clinical. Conclusion The Qingre Runzao oral liquid has significant antitussive and antiasthmatic effects. The contemplated daily dosage is safe in clinical applications.