摘要
目的:探讨人类疱疹病毒8型(HHV-8)ORF50启动子区在经典型卡波西肉瘤(经典型KS)与艾滋相关型卡波西肉瘤(AIDS-KS)中甲基化状态的差异。方法:采用甲基化特异性PCR(MSP)检测经典型KS与AIDS-KS的甲基化状态,分析检测结果。结果:37例经典型KS中14例发生甲基化,甲基化阳性率为38%;18例AIDS-KS中5例发生甲基化,甲基化阳性率为28%。两者甲基化状态的差异无统计学意义(P>0.05)。结论:经典型KS与AIDS-KS中HHV-8 ORF50启动子的甲基化状态无明显的差异,HHV-8ORF50启动子的甲基化状态可能与HIV感染无关。
Objective：To study the difference between the classical Kaposi＇s sarcoma and AIDS related Kaposi＇s sarcoma（AIDS-KS） in the promoter region of human herpesvirus 8（HHV-8）.Methods：To use the methylation specific PCR（MSP） to detect the methylation status between the classical Kaposi＇s sarcoma and AIDS-KS.Results：14（38%） of 37 cases of classical Kaposi＇s sarcoma were methylated,while 5（28%） of 18 cases AIDS-KS were methylated,（P0.05）.Conclusion：There was no significant difference between the classical Kaposi＇s sarcoma and AIDS-KS of methylation status in the promoter region of HHV-8 ORF50,maybe the infection of HIV had nothing to do with the status of methylation in the promoter region of HHV-8 ORF50.
出处
《现代肿瘤医学》
CAS
2013年第7期1434-1436,共3页
Journal of Modern Oncology
基金
国家自然科学基金地区科学基金项目(编号:81260311)
新疆维吾尔自治区人民医院院内项目(编号:20120101)
