黄芪三参饮不同剂量及作用时限对小鼠小肠隐窝效应的研究

Effects of different doses and different time of Huang Qi San Shen Yin on small intestinal crypts of mouse

目的:观察黄芪三参饮(Huang Qi San Shen Yin,HQSSY)不同剂量及作用时限对小鼠小肠隐窝的效应。方法:选取健康昆明种小鼠100只,随机分为2组,50只连续给药2周,50只连续给药4周。两组又均分为空白组、低、中、高倍剂量组。实验组给予不同剂量的黄芪三参饮,而空白组予以等量的蒸馏水。在连续给药2周及4周后,检测各组小鼠血常规及小肠隐窝,分析最佳服药时间及剂量。结果:各组白细胞、红细胞、血红蛋白及血小板均无明显差异(P>0.05)。小肠隐窝数在2周给药组中,中倍剂量组与空白组存在统计学差异(P<0.05),其他各剂量浓度则无统计学差异(P>0.05);4周给药组中各剂量浓度组与空白组相比,均无统计学差异(P>0.05)。相同剂量浓度时,服2周中药组与服4周中药组间小鼠存在统计学差异(P<0.05),中倍剂量浓度组存在非常显著性差异(P<0.01)。说明服2周中药中倍剂量组对促进隐窝细胞增殖作用最明显。结论:HQSSY对小鼠小肠隐窝有促再生作用,对放射性肠炎可能具有保护作用,为后续研究的药物剂量和服药时限提供依据。

Objective：To investigate the effects of different doses and different time of Huang Qi San Shen Yin（HQSSY） on small intestinal crypts of mouse.Methods：Total of 100 healthy Kunming mice were randomly divided into 2 groups,50 mice were treated for 2 weeks and 50 mice were treated for 4 weeks.Both 2 groups were divided into control group（n=14）,low dose group（n=12）,middle dose group（n=12） and high dose group（n=12）.The control group was given a gavage of distilled water of the same volume with the drug,and the three experimental groups were given different doses of HQSSY,which were 1,2 and 4 times of human dosage respectively.At the end of the experiment,routine blood and small intestinal crypts of mice were measured.Results：WBC,RBC,HB and PLT in each group were not significantly different（P0.05）.Comparison of small intestinal crypts.In 50 mice after 2 weeks of treatment,there were statistical difference between the middle dose group and the control group（P0.05）;Others had no statistical significance（P0.05）.In 50 cases after 4 weeks of treatment,compared with the control group,each dose concentration group had no statistical difference（P0.05）.In the same dose concentration,there was statistical difference between the group of 2 weeks treatment and the group of 4 weeks treatment（P0.05）,while the middle dose concentration groups had very significant difference（P0.01）,which indicated that the group of 2 weeks treatment in middle dose HQSSY promote crypts cell proliferation obviously.Conclusion：HQSSY can promote the regeneration of small intestinal crypts of mice and prevent radioactive enteritis.This research can provide reliable basis for follow-up study.