塞来昔布对卵巢癌细胞SKOV3生长与侵袭能力的抑制效应

Inhibitory effect of celecoxib on proliferation,invasion and VEGF expression of ovarian carcinoma SKOV3 cells

目的:探讨环氧化酶-2(COX-2)选择性抑制剂塞来昔布(celecoxib)对卵巢癌细胞增殖、侵袭能力、血管内皮生长因子(VEGF)表达的影响。方法:SKOV3细胞经塞来昔布处理后,以MTT比色法测定各组细胞增殖水平,以细胞侵袭实验检测细胞侵袭转移能力,RT-PCR及ELISA检测SKOV3细胞VEGF mRNA及蛋白的表达水平。结果:与对照未加药组相比,不同浓度塞来昔布可有效抑制SKOV3细胞的增殖与侵袭能力,同时显著下调细胞VEGF在mRNA及蛋白水平的表达水平。上述效应均呈明显剂量效应关系,组间差异均有统计学意义(P<0.05)。结论:塞来昔布对卵巢癌SKOV3细胞的增殖、侵袭能力及VEGF表达均有明显的抑制作用。

Objective：To investigate the effects of cyclooxygenase-2（COX-2） inhibitor celecoxib on proliferation,invasion,VEGF expression in nasopharyngeal carcinoma SKOV3 cells.Methods：SKOV3 cells were treated with celecoxib at various concentrations.MTT assay and invasion assay were performed to observe the inhibitory activity of celecoxib on SKOV3 cells,and VEGF expression was analyzed by RT-PCR and ELISA.Results：Treatment of celecoxib significantly suppressed the capacity of proliferation,invasion and down-regulated the VEGF mRNA and protein expression of SKOV3 cells,in a dose-dependent manner（P0.05）.Conclusion：Celecoxib has a significant inhibitory activity on cell proliferation,invasion and VEGF expression in ovarian carcinoma SKOV3 cells.These findings implicate that application of celecoxib may be an effective strategy for the therapy of ovarian carcinoma.