C/EBP_α基因对维吾尔族宫颈鳞癌组织细胞增殖的影响

Effect of C/EBP alpha gene on cellular proliferation in Uygur patients with cervical squamous cell carcinoma

目的探讨CCAAT/增强子结合蛋白α(CCAAT/enhancer binding protein alpha,C/EBPα)在宫颈癌组织中的表达水平及其对子宫颈鳞癌组织细胞增殖的影响。方法 7例宫颈鳞癌患者的新鲜标本(宫颈鳞癌1组)与5例正常妇女的新鲜标本(对照组),采用RT-PCR检测C/EBPαmRNA与Ki-67mRNA表达情况;35例宫颈鳞癌患者的石蜡标本(宫颈鳞癌2组)与35例慢性宫颈炎患者石蜡标本(宫颈炎组),采用免疫组织化学方法检测C/EBPα与Ki-67蛋白表达情况。结果对照组C/EBPαmRNA表达水平高于宫颈鳞癌1组;Ki-67mRNA低于宫颈鳞癌1组;宫颈炎组C/EBPα蛋白表达水平高于宫颈鳞癌2组(P<0.01),Ki-67蛋白表达水平低于宫颈鳞癌2组(P<0.05);宫颈鳞癌2组C/EBPα蛋白与Ki-67蛋白表达呈负相关(r=-0.259,P<0.05)。结论宫颈鳞癌组织中C/EBPα基因mRNA和蛋白表达均降低;C/EBPα基因对子宫颈鳞癌组织细胞的增殖起抑制作用。

Objective To explore the expression of CCAAT/enhancer binding protein alpha（C/EBPα） in cervical carcinoma tissue and its effect on cellular proliferation in Uygur patients with cervical squamous cell carcinoma.Methods The expressions of C/EBPα gene and Ki-67 were detected by using real-time PCR technique in 7 cases of fresh cervical cancer tissue（cancer group 1） and 5 cases of fresh normal tissue（normal group）,and by using immunohistochemistry in 35 cases of paraffin cervical cancer tissue（cancer group 2） and 35 cases of paraffin chronic cervicitis tissue（cervicitis group）.Results The level of C/EBPα gene mRNA was higher and the level of Ki-67 gene mRNA was lower in control group than that in cancer group 1（P0.01）.The level of C/EBPα protein was higher,and the level of Ki-67 protein was lower in cervicitis group than that cancer group 2（P0.05）.The expression of C/EBPα was negatively correlated with the expression of Ki-67（r=-0.259,P0.05）.Conclusion The expressions of C/EBPα gene mRNA and protein in cervical cancer tissue decrease,and C/EBPα gene inhibits the cellular proliferation in cervical squamous cell carcinoma tissue.