吸烟对正常男性和弱精子症男性精子DNA完整性的影响

Smoking affects sperm DNA integrity in normozoospermic and asthenozoospermic men

目的探讨吸烟对正常男性和弱精子症男性精子DNA完整性的影响。方法采用病例-对照配对法,通过染色质扩散法测定了重度吸烟和从不吸烟的164例正常男性和120例弱精子症男性的精子DNA碎片指数(DFI)。结果重度吸烟者相比非吸烟者DFI显著升高(P<0.01),其值分别为17.8和14.6。弱精子症患者与正常男性相比DFI显著升高(P<0.001),其值分别为19.2和14.9。在正常精子男性中,重度吸烟者比非吸烟者DFI显著升高(P<0.05),而在弱精子症患者中,重度吸烟者也比非吸烟者的DFI显著升高(P<0.05)。结论弱精子症患者比正常男性的DNA完整性降低。重度吸烟对于正常男性和弱精子症患者都是一个损伤DNA的危险因子。吸烟可能与其它病理因素共同影响精子的DNA完整性。

Objective： To investigate the impact of smoking on sperm DNA integrity in patients with normozoospermic and asthenozoospermic. Methods： This paired case-control study was conducted in heavy smoking or non-smoking patients including 164 normozoospermic and 120 asthenozoospermie patients. Sperm DNA integrity presented by the DNA fragmentation index（DFI）was assayed by sperm chromatin dispersion（SCD）method. Results： The DFI of heavy smokers was significantly higher than that of non-smokers（P〈0. 001）, with values of 17.8 and 14.6 respectively. The asthenozoospermic patients had higher I）F1 compared to the normozoospermic patients （P 〈 0. 001）, with values of 19.2 and 14.9 respectively. The heavy-smoking normozoospermic patients had significantly higher DFI than the non-smoking normozoospermic patients （P〈0.05）. The heavy-smoking asthenozoospermic patients also had significantly higher DFI than non-smoking asthenozoospermic patients（P〈0.05）. Conclusions： Asthenozoospermic patients have lower sperm DNA integrity than normozoospermic patients. Heavy smoking can impair sperm DNA integrity in both normozoospermic and astheznozoospermic patients. Smoking is a risk factor for sperm DNA integrity.