慢性压迫背根神经节对成年雄性大鼠脊髓背角P2X3受体表达的影响

Effect of Chronic Compression of Dorsal Root Ganglion on P2X3 Receptor Expression in Spinal Dorsal Horn of Adult Female Rat

目的观察神经病理性疼痛下脊髓背角内P2X3受体的表达情况。方法将成年雄性大鼠随机分为3组(每组n=6),分别为空白组、假手术组、慢性压迫背根神经节(CCD)组。通过压迫L4和L5背根神经节建立大鼠神经病理性疼痛模型。术后第7天分别测定各组大鼠的机械痛阈值,并用RT-PCR和蛋白印迹的方法测定大鼠患侧脊髓背角中P2X3基因mRNA和蛋白的表达水平。结果 CCD组大鼠表现出机械性痛觉过敏增强和疼痛阈值降低,同时CCD组大鼠术侧脊髓背角mRNA和蛋白的表达明显高于假手术组(P<0.01)。假手术组和空白组比较差异无显著性。结论 CCD诱导大鼠患侧脊髓背角P2X3受体的表达增高。

Objective To observe P2X3 receptor expression in spinal dorsal horn under neuropathic pain. Methods Aduh female rats were randomly divided into 3 groups（n =6 each） ：Control group,Sham operation group and chronic compression of dorsal root ganglion group（ CCD group）. Neuropathic pain rat model by compressing IA and I5 dorsal mot ganglion was used. Mechanical pain threshold was measured on 7 day after the CCD operation, P2X3 mRNA and protein expression level in spinal dorsal horn were evaluated by RT-PCR and Western blotting. Results CCD group manifested mechanieal hyperalgia and reduced pain threshold. Simultaneously expressions of mRNA and protein of P2X3 receptor in spinal dorsal horn were significantly increased in CCD group compared with those in sham operation group（P 〈0.01）. There was no dift^rence hetween sham operation group and control group. Conclusion CCD induced P2X3 receptor over expression in spinal dorsal horn of rats.