子痫前期与胎儿生长受限患者激素相关基因的表达水平

The expression level of hormone related genes of preeclampsia and fetal growth restriction patients

目的应用基因芯片技术检测子痫前期及胎儿生长受限患者和正常妊娠胎盘组织差异表达基因,探索两种疾病的病因。方法用RMA的方法对芯片原始数据归一化。采用斯坦福大学的SAM(Significance Analysis of Microarrays)检验进行显著性检验。然后对显著性差异的基因做基因富集分析。结果子痫前期组与正常孕妇组比较经SAM检验找出差异表达的111个基因,与激素的分泌、转运、调节等过程高度相关。胎儿生长受限组与正常孕妇组比较经SAM检验后找出差异表达的63个基因,其中3个与激素相关的基因。结论子痫前期和胎儿生长受限在与激素相关的基因表达水平上都与正常孕妇存在差异,提示子痫前期和胎儿生长受限在与激素相关基因的表达水平上具有一定的相关性。

Objective To investigate the new etiology of preeclampsia and fetal growth restriction by testing the placental gene expression difference among the two kinds of diseases and normal pregnancy by microarray.Methods RMA was used to normalization the initial data.SAM of the Stanford University was used to test significance.And then enrichment gene list was analyzed in significant genes.Results A total of 111 differently-expressed genes,which were highly related to secretion,transport,regulation of hormone,were identified in placental tissue of preeclampsia,and 63 differently-expressed genes were found in placental tissue of fetal growth restriction,including 3 genes related to hormones.Conclusion There are different hormone-related genes expression levels in preeclampsia and fetal growth restriction to the normal pregnant women,which suggests that there are certain correlation with preeclampsia and fetal growth restriction in the hormone-related genes expression levels.