碱性成纤维细胞生长因子对人牙髓细胞生物学活性的影响

The effect of basic fibroblast growth factor on the biologic activity of human dental pulp cells

目的:研究碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对体外培养的人牙髓细胞(human dental pulp cells,HDPCs)生物学活性的影响。方法:常规组织块培养法获得HDPCs,采用四唑盐比色(MTT)法、ALP活性检测法观察不同浓度bFGF(0.1、1、10、100μg/L)对体外培养的HDPC粘附性、伸展性以及ALP活性的影响。结果:不同浓度bFGF均可提高HDPCs粘附性和伸展性,与对照组相比P<0.05;不同浓度bFGF与HDPCs共同培养一定时间后,均能显著抑制其ALP活性(P<0.05);并且浓度越高,抑制作用出现的时间越早,100μg/L组在培养第1天时与对照组相比P<0.05。结论:0.1～100μg/L范围内不同浓度bFGF均能促进HDPCs的粘附性和伸展性,并可抑制HDPCs的ALP活性。

AIM： To investigate the effects of basic fibroblast growth factor （bFGF） on the biologic activity of human dental pulp cells （HDPCs） in vitro. METHODS ： HDPCs were obtained from extracted human premolar and cultured in DMEM medium with 10% fetal bovine serum （FBS）. The cells were treated by bFGF at 0. 1 μg/L, 1μg/L, 10μg/L and 100 μg/L respectively. Ceil attachment assay and spreading assay were conducted. Alkaline phosphatase （ALP） activity of the ceils was examined by enzyme dynamics method. RESULTS： bFGF at 0. 1 μg/L 1 μg/L, 10 μg/L and 100 μg/L promoted the attachment ability and the spreading rate of HDPCs （ vs control, P 〈 0.05 ）, and suppressed ALP activity of the ceils （vs control P 〈 0.05 ） in a dose -and time-dependent manner. CONCLUSION： bFGF can increase the attachment ability and spreading rate , and decrease ALP activity of of HDPCs.