黑曲霉产木聚糖酶发酵条件的研究

The Study on Xylanase fermentation by Aspergillus niger sp.

正交设计试验结果表明 ,黑曲霉 (Aspergillusnigerm1 2 )产木聚糖酶活力达76 .6 0u/ml,合适的产酶发酵条件如下 ,培养基 ( g/L) :麸皮 40 ,尿素 6 .6 7,KH2 PO41 .0 ,MgSO4·7H2 O 0 .5 ,NaCl 0 .3,Tween 80 3.0 ,CaCO3 2 .0 ,2 8℃ ,1 2 0r/min水浴振荡培养 5 .5d。

Through orthogonal experiment,suitable conditions for the xylanase fermentation by Aspergillus niger sp.m12 were obtained.The medium (g/L) contained wheat bran 40.0,urea 6.7,KH 2PO 4 1.0,MgSO 4·7H 2O 0.5,NaCl 0.3,Tween 80 3.0,CaCO 3 2.0; 1.0×10 7 spores were inoculated in 50 ml Erlenmeyer flask with a water rocker (120 r/min,28℃) which stirred for 5.5 days.The enzyme activity of the culture filtrate was 76.60 u/ml.