脂肪酶产生菌的筛选及不对称水解合成S-(+)-萘普生

Screening of lipase producting strain and synthesis of S-(+)-naproxen by asymmetric hydrolysis

从 1 6个土样中分离获得一株脂肪酶产生菌E 5 3# ,该菌可优先水解S ( +) 萘普生甲酯为S ( +) 萘普生 ,ee值可超过 87%。该菌经初步鉴定为芽孢杆菌。最佳产酶培养基为葡萄糖 0 .5 %、蛋白胨 0 .5 %、酵母膏 0 .2 %。 0 .5 %的橄榄油 ,能诱导脂肪酶的大量产生。

A bacterium, Bacillus E?53 was screened from soil, which preferentially hydrolyzed S (+) isomer of the racemic naproxen methyl ester to produce S (+) naproxen. The strain showed higher specificity of resolution about 87% ee. The optimal medium for producing lipase contained 0.5% of glucose, 0.5% of peptone and 0.2% of yeast extract. 0.5% of olive oil could induce lipase production.