摘要
The protoplasts of auxotrophic mutant monokaryon of Pleurotus. sajor-caju was used as recipient parenL the protoplasts of wild dikareon of Lentinus edodes were heat-inactivated and used as nuclear donor parent. The fusants of two parents showed some their individual characteristics and physiological difference between themselfs. Thrugh protoplast re-isolation and reversion of one randomly selective fusant, two parents had been recovered and the donor parent achieved some new physiological characteristics such as faster mycelial growth and earier fruiting.This experiment result showed that the idea and the method of polarized protoplast fusion used in this study may be used as routine procedure for strain improvement in adible fungi.
The protoplasts of auxotrophic mutant monokaryon of Pleurotus. sajor-caju was used as recipient parenL the protoplasts of wild dikareon of Lentinus edodes were heat-inactivated and used as nuclear donor parent. The fusants of two parents showed some their individual characteristics and physiological difference between themselfs. Thrugh protoplast re-isolation and reversion of one randomly selective fusant, two parents had been recovered and the donor parent achieved some new physiological characteristics such as faster mycelial growth and earier fruiting.This experiment result showed that the idea and the method of polarized protoplast fusion used in this study may be used as routine procedure for strain improvement in adible fungi.
出处
《菌物系统》
CSCD
北大核心
2000年第3期413-415,共3页
Mycosystema
关键词
凤尾菇
香菇
原生质体
非对称融合
杂交育种
Pleurotus sajor-caju, Lentinus edodes, Protoplast, Polarized fusion
