广西产三种地钱RAPD分子鉴定初步研究

A preliminary study on the identification of three kinds of marchantia in Guangxi by RAPD molecular markers

目的:建立广西产地钱(Marchantia polymorpha L.)拳卷地钱(Marchantia convoluta gao et chang.)和粗裂地钱(Marchantia paleacea Bertol.)的分子鉴别方法。方法:利用RAPD(random amplified polymorphic DNA)技术。结果:采用改良的CTAB法提取地钱、拳卷地钱、粗裂地钱3种植物的总DNA均适用于22引物对供试材料DNA进行随机扩增,共扩增出145条谱带,多态性条带128条,占扩增总条带的88.3%,平均多态性百分率为89.8%;三种地钱遗传距离为0.3713-0.4107、Nei′s遗传一致度为0.5893-0.6287。结论:RAPD技术对广西产地钱、拳卷地钱、粗裂地钱进行分子鉴定是有效的。RAPD分析。

Objective： To establish a molecular identification method to identify the Marchantia polymorpha linn.Marchantia convoluta gao et k.c.zhang and Marchantia paleacea bertol.in Guangxi.Methods： the RAPD molecular makers.Results： the modified CTAB method was applicable to analysis the total DNA of the Marchantia polymorpha L.;Marchantia convolute Gao et Chang.and Marchantia paleacea Bert.in Guangxi,145 bands were amplified totally by 22 pair primers,of which 128.Bands（88.3%） were polymorphic and average polymorphism rate was 89.8%;genetic distance was 0.3713-0.4107,Nei＇s genetic identity was 0.5893-0.6287 Conclusion： The RAPD markers method is available to identify the Marchantia polymorpha linn.Marchantia convoluta gao et k.c.zhang and Marchantia paleacea bertol.in Guangxi