红豆草耐盐愈伤组织的筛选及植株再生(英文)

Selection for salt tolerance in tissue culture of sainfoin and recovery of plants from NaCl tolerant callus

将红豆草种子在含１．２％ ＮａＣｌ的ＭＳ培养基上萌发以消除盐敏感的幼苗，把存活的幼苗下胚轴切段在含１ ｍ ｇ／Ｌ２，４－Ｄ、０．５ ｍ ｇ／Ｌ ６－ＢＡ 及１．２％ ＮａＣｌ的ＭＳ培养基上诱导愈伤组织。通过连续筛选得到可耐受１．８％ ＮａＣｌ的愈伤组织。在有０．２ ｍ ｇ／ＬＮＡＡ 和１ｍ ｇ／Ｌ ＩＡＡ 存在下该愈伤组织分化出芽，待幼苗长至３ ｃｍ 左右时转到含２ ｍ ｇ／Ｌ ＮＡＡ 或ＩＢＡ 的１／２ ＭＳ培养基上生根。与对照愈伤组织相比。

Seeds of sainfoin were germinated in MS medium plus 1.2% NaCl to eliminated the salt sensitive seedlings and the hypocotyl segments of survived seedlings were used to induce callus on MS medium containing 1 mg/L 2,4 dichlorophenoxyacetic acid,0.5 mg/L 6 benzyladenine and 1.2% NaCl.The organigenetic callus tolerating 1.8% NaCl was obtained by continuos selection on NaCl containing medium.Shoots regenerated from the selected callus on MS medium containing 0.2 mg/L α naphthaleneacetic acid and 1 mg/L indole 3 butyric acid.Roots were induced from 3 cm high shoots on half strength MS medium plus 2 mg/L α naphthaleneacetic acid or indole 3 butyric acid.The selected tolerant callus and the reinitiated callus from the regenerated plant showed a higher tolerance to osmotic stress than the nonselected callus.