白木千胚性愈伤组织长期继代培养中的分化能力及染色体稳定性研究

Studies on differentiation potential and chromosome stability of embryogenic callus in subcultures of Picea meyeri Rehd.et Wils.

白木千（Ｐｉｃｅａ ｍ ｅｙｅｒｉ Ｒｅｈｄ．ｅｔ Ｗｉｌｓ．）的胚性愈伤组织在ＭＳ＋ ２，４－Ｄ １ ｍ ｇ／Ｌ＋ ＫＴ １ｍ ｇ／Ｌ的培养基上继代３ 年，增殖能力和分化潜力仍保持在原来的水平，没有明显降低的趋势。但随着继代时间的增长，胚性愈伤组织内有些细胞的染色体数目发生了无规律的变化，而再生植株根尖细胞染色体数目比较稳定（２ｎ＝ ２８）。

Embryogenic callus of Picea meyeri Rehd.et Wils.cultured on MS+2,4 D 1 mg/L+KT 1 mg/L remained high growth rate and differential ability through 3 year subcultures.There were variations in number of chromosomes in embryogenic callus cells.But the number of chromosomes in root tip cells of regenerated plantlets was relatively stable (2n=28).