烟草未授粉子房胚状体诱导的研究

Studies on the induction of embryoid from unpollinated ovaries of Nicotiana tabacum L.

对烟草（Ｎｉｃｏｔｉａｎａ ｔａｂａｃｕｍ Ｌ．）未授粉子房胚状体的诱导进行了研究，结果发现，胚状体是单细胞起源，起源于大孢子或卵细胞，接种发育早期的子房，胚状体起源于大孢子；接种发育晚期的子房，胚状体起源于卵细胞；接种发育中期的子房，胚状体多起源于大孢子，少数起源于卵细胞。不同的基因型，蔗糖浓度及光照强度等对胚状体诱导率的影响亦有不同。

Induction of embryoid from unpollinate ovaries of Nicotiana tabacum L.was investigated in this thesis.The results obtained were summarized as follows.Embryoid was originated from single cell,either megaspore or egg cell.Ovary inoculated at early stage produced embryoid from megaspore,while ovary inoculated at late stage produced embryoid from egg cell.When ovaries were plated at middle stage,embryoid originated more from megaspore than from egg cell.The ability of embryoid production of various genotypes,various concentrations of sucrose,various light intensity changed greatly.