慢性阻塞性肺疾病急性加重期患者外周血组蛋白去乙酰化酶2的表达及临床意义

Histone deacetylase 2 levels in peripheral blood of patients with acute exacerbation of chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺疾病(COPD)急性加重期患者外周血组蛋白去乙酰化酶2(histone deacetylase 2,HDAC2)的表达及其与C反应蛋白(CRP)、降钙原素(PCT)的关系。方法选择67例COPD急性加重期患者,根据病情分为轻、中、重度3组,均行肺功能检查。选择同期体检中心20例健康体检者为对照组。ELISA双抗体夹心法测定HDAC2表达水平,免疫比浊法测定炎症蛋白CRP、PCT浓度。结果随着病情的加重,外周血炎症蛋白CRP、PCT浓度显著升高,而HDAC2浓度呈逐渐下降趋势,各组间的差异均有统计学意义(均P<0.01)。HDAC2表达水平和炎症蛋白PCT浓度呈负相关(P<0.05)。结论 HDAC2可能是COPD急性加重期患者炎症反应的负调控因子。

Objective To investigate histone deacetylase 2 （HDAC2） levels in peripheral blood of patients with acute ex-acerbation of chronic obstructive pulmonary disease （COPD）. Methods Sixty-seven patients with COPD in acute exacerbation were enrol ed in the study. Lung function tests were performed to evaluate disease severity and patients were classified as mild, moderate and severe groups. Twenty healthy subjects served as controls. The HDAC2 levels were measured by ELISA. The in-flammatory proteins C-reactive protein （CRP） and procalcitonin （PCT） were measured by immunoturbidimetric assay. Results The CRP and PCT levels were increased in COPD patients as compared to healthy controls. The HDAC2 levels in mild, moderate and severe groups were 6.93±1.25, 6.42±1.46 and 3.79±1.12, respectively （P〈0.01）. HDAC2 level was negatively correlated with inflammatory protein PCT （P〈0.05）. Conclusion HDAC2 levels are decreased in peripheral blood of COPD patients and correlated with the severity of disease, indicating that it may be a negative regulator of inflammatory response in patients with COPD.