中国石榴(Punica granatum L.)居群遗传多样性

Genetic diversity of pomegranate(Punica granatum L.) population in China by RAMP markers

为了更有效地保护和利用石榴种质资源,利用RAMP标记对中国7个石榴主产区的46个石榴品种进行遗传分析。结果显示:14对RAMP引物共扩出127条DNA条带,其中113条显示多态性,多态位点百分率(P)为88.98%;7个石榴居群遗传多样性依次为:山东居群>陕西居群>云南居群>河南居群>安徽居群>四川居群>新疆居群;7个居群间的基因分化系数(Gst)为0.183 0;群间基因流的估测值(Nm)为2.231 5。表明中国石榴具有较大的遗传变异及丰富的遗传多样性,居群间存在较强的基因流。

To protect and utilize pomegranate resources in China, random amplified microsatellite polymorphism （RAMP） technique was used to analyze the genetic relationships among 46 pomegranate genotypes collected from seven major production areas in China. A total of 127 bands were amplified by fourteen pairs of high polymorphism primers, among which 113 （88.98%） were polymorphic loci. The genetic diversity of seven pomegranate populations followed the order of Shandong 〉 Shannxi 〉 Yunnan 〉 Henan 〉 Anhui 〉 Sichuan 〉 Xinjiang. The gene differentiation coefficient （Gst） of seven populations was 0. 183 0, and the gene flow （Nm） was 2. 231 5. These statistics indicated that the present pomegranate resources in China had high genetic variation and genetic diversity, and a strong gene flow existed among these populations.