IL-18基因启动子区-137G/C多态性与急性脑梗死关系的研究

Correlation between Polymorphism of Promoter Region of Interleukin-18 Gene at Position-137 G/C and Acute Cerebral Infarction

目的探讨白细胞介素-18(IL-18)基因启动子区-137G/C多态性及血清IL-18水平与急性脑梗死的相关性。方法用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测急性脑梗死患者153例及健康对照组114例的IL-18基因启动子区-137G/C多态性,用酶联免疫吸附法(ELISA)检测两组血清IL-18浓度。结果与对照组相比,急性脑梗死组IL-18基因启动子区-137G/C多态性各基因型分布及等位基因频率差异均有统计学意义(P<0.05),携带C等位基因的个体急性脑梗死的相对风险度要比未携带者增加1.672倍。急性脑梗死组血清IL-18浓度明显高于对照组(P<0.05),急性脑梗死组携带C等位基因的个体的血清IL-18浓度明显高于未携带者(P<0.05)。结论 IL-18基因启动子区-137G/C多态性与急性脑梗死的发病具有相关性,携带C等位基因可促使个体高度表达IL-18,通过影响IL-18血清浓度从而对急性脑梗死的发生发展产生作用,增加急性脑梗死的发病风险。

Objective To investigate the correlation between polymorphism of promoter region of interleulin-18 （IL-18） gene at position -137G/C and serum IL-18 level with acute cerebral infarction. Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to detect the polymorphism of promoter region of IL-18 gene at position -137G/C in 153 cases of acute cerebral infarction and 114 normal controls,and enzyme-linked immunosorbent assay （ELISA） was used to test serum IL-18 level in the two groups. Results There was significant difference in the genotype distribution and allele frequency of polymorphism of promoter region of IL-18 gene at position -137G/C between acute cerebral infarction group and normal controls（P 〈0.05）. The risk of acute cerebral infarction to the carriers with C allele genetype increased to 1. 672 times as compared with the cases without C allele genotype. The serum IL-18 level in the acute cerebral infarction group was significantly higher than the controls（P 〈0.05） ,and the carriers with C allele genotype showed significantly higher serum IL-18 levels compared with the cases without C allele genotype in acute cerebral infarction group（P 〈0.05）. Conclusion The polymorphism of promoter region of IL-18 gene at position -137G/C is associated with the onset of acute cerebral infarction. The C allele genotype may lead to a high expression of IL-18 ,which plays a role on the occurrence and development of acute cerebral infarction by affecting serum IL-18 level,and may increase the risk of acute cerebral infarction.