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1,25-二羟维生素D_3对白血病6T-CEM细胞株作用的体外研究 被引量：5

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摘要 目的研究1,25-二羟维生素D3[1,25(OH)2D3]对人白血病6T-CEM细胞株凋亡及维生素D受体(VDR)蛋白表达的影响。方法在体外用不同浓度的1,25(OH)2D3作用于6T-CEM。末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记染色法(TUNEL)检测细胞晚期凋亡的变化,免疫细胞化学法检测VDR蛋白表达。结果不同浓度的1,25(OH)2D3作用后可促进6T-CEM细胞的凋亡,呈剂量和时间依赖性(P<0.05)。1,25(OH)2D3作用前后6T-CEM细胞均表达VDR蛋白,药物作用后VDR蛋白表达上调。结论 1,25(OH)2D3在一定浓度范围内可诱导6T-CEM细胞凋亡,使VDR蛋白表达上调。
作者 郑敏 何云燕 罗建明
机构地区 广西柳州市妇幼保健院儿内科 广西医科大学第一附属医院儿科
出处 《广西医学》 CAS 2013年第6期730-731,共2页 Guangxi Medical Journal
关键词 白血病细胞株 维生素D受体 1 25-二羟维生素D
分类号 R733.71 [医药卫生—肿瘤]
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参考文献9

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同被引文献55

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广西医学
2013年 第6期

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