摘要
目的研究旋毛虫感染对过敏性哮喘小鼠血清TIgE、IL-4、IL-5的影响。方法随机将20只BALA/c雌性小鼠分为4组,每组5只,Ⅰ组为空白对照组,Ⅱ组为单纯过敏性哮喘组,Ⅲ组为感染旋毛虫后哮喘组,Ⅳ组为单纯感染旋毛虫组。首先,将200～300条旋毛虫囊包幼虫经口感染Ⅲ、Ⅳ组小鼠,建立旋毛虫感染模型。28 d后,用卵清白蛋白分别对Ⅱ组和Ⅲ组小鼠进行致敏激发,建立过敏性哮喘模型。56 d后,取小鼠血清,用ELISA法检测TIgE、IL-4、IL-5水平。结果 4组小鼠总IgE水平分别为:(61.79±25.79)ng/ml、(437.08±75.68)ng/ml、(251.64±107.27)ng/ml和(446.12±74.32)ng/ml;IL-4水平分别为:(136.49±31.17)pg/L、(209.80±21.57)pg/L、(162.79±17.69)pg/L和(182.71±12.08)pg/L;IL-5水平分别为:(7.31±1.86)ng/L、(16.00±2.28)ng/L、(9.24±1.73)ng/L和(11.41±2.11)ng/L;与Ⅱ组相比较,Ⅲ组血清中总IgE、IL-4及IL-5水平明显降低(P<0.05)。结论旋毛虫感染可以抑制过敏性哮喘小鼠血清TIgE、IL-4及IL-5的表达。
Objective To study the effect of Trichinella spiralis infection on total IgE,IL-4 and IL-5 in serum of the mice with allergic asthma.Methods Twenty female BALB / c mice were randomly divided into four groups of five mice each,including Ⅰ as control group,Ⅱ as allergic asthma group,Ⅲ as asthma followed by Trichinella spiralis infection,Ⅳ as Trichinella spiralis infected group.Firstly,the animals in group Ⅲ and Ⅳ were infected with 200-300 Trichinella spiralis muscle larvae.Twenty-eight days later,ovalbumin（OVA） was used to induce the allergic asthma for mice in group Ⅱ and Ⅲ,in order to establish asthma animal model.After 56 days,all mice were sacrificed,levels of TIgE,IL-4 and IL-5 in serum were measured by ELISA.Results The TIgE levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were（61.79 ± 25.79） ng / ml,（437.08 ± 75.68） ng / ml,（251.64 ± 107.27） ng / ml and（446.12 ± 74.32） ng / ml,respectively.The IL-4 levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were（136.49 ± 31.17） pg / L,（209.80 ± 21.57） pg / L,（162.79 ±17.69） pg/L and（182.71 ±12.08） pg/L,respectively.IL-5 levels in groupⅠ,Ⅱ,Ⅲ and Ⅳ were（7.31 ±1.86） ng/ L,（16.00 ± 2.29） ng / L,（9.24 ± 1.73） ng / L and（11.41 ± 2.11） ng / L,respectively.Compared with group Ⅱ,the TIgE、IL-4 and IL-5 levels in group Ⅲ decreased（P〈0.05）.Conclusions Trichinella spiralis infection can inhibit expression of the TIgE,IL-4 and IL-5 in serum of mice with allergic asthma.
出处
《武警医学》
CAS
2013年第6期502-504,共3页
Medical Journal of the Chinese People's Armed Police Force
