摘要
以预先驯化的菌群和活性污泥作为起始接种物用于生物滴滤池(BTF)中,研究评估了BTF去除苯、甲苯、乙苯和二甲苯混合气体(BTEX)的性能,并利用变性梯度凝胶电泳(DGGE)技术分析了微生物群落结构的变化.结果表明,BTF能在短时间内得到驯化,填料附着的生物量从第10 d的5.7 mg.g-1迅速增加至第30 d的112 mg.g-1.BTF能同时有效去除混合BTEX中的各组分,在进气负荷和停留时间分别为269.7 g.(m3.h)-1和39 s时,可获得的最大去除能力为216.6 g.(m3.h)-1.DGGE图谱表明,BTF中优势微生物种群主要来源于富集菌群,微生物群落结构随着运行时间发生变化,但在BTF上下空间分布较为均匀.
The pre-acclimated microbial consortium and the activated sludge were used as start inoculums of a bench-scale biotrickling filter (BTF). The performance of the biotrickling filter on the removal of BTEX mixture was evaluated, and the changes in the bacterial community structure of the BTF were analyzed by PCR-DGGE technique. The results showed that the BTF could be acclimated within a short time, the biomass that adhered to the surface of packing materials increased rapidly from 5.7 mg·g-1 at 10th day to 112 mg·g-1 at 30th day. BTF could simultaneously remove all components of the BTEX mixture efficiently. The maximum removal capacity of the BTF was 216.6 g·(m3·h)-1, which was achieved with an inlet loading rate of 269.7 g·(m3·h)-1 and an empty bed retention time (EBRT) of 39 s. DGGE analysis indicated that the dominant microorganisms may be derived from the pre-acclimated microbial consortiums rather than the activated sludge. Although the bacterial community changed with run time, the spatial distribution was very uniform.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2013年第7期2552-2559,共8页
Environmental Science
基金
国家自然科学基金项目(31270169)
广东省科技计划项目(2010A030200021
2011A030700002)
关键词
生物滴滤池
BTEX
DGGE
群落结构
菌群
biotrickling filter(BTF)
BTEX
DGGE
community structure
microbial consortium