探讨乙型肝炎病毒子宫内传播的病毒群演变

Variant of HBV intransmission from mother to fetus

以 6名HBsAg、HBeAg均阳性的女性携带者及其子宫内感染HBV的 6例胎儿为对象 ,探讨母儿间病毒群的演变。检测母儿所携HBVS区 45 1～ 6 6 0、C区 2 0 2 2～ 2 30 1位核苷酸序列。结果表明了母儿间同源性 98%～1 0 0 % ,检出 5 30、5 46、5 81位点变异致使 1 2 6、1 31、1 43位氨基酸替代。 2对母儿C区同源性 1 0 0 % ,其中一对母儿均有原型株、变异株混合感染。结论子宫内感染大部分母亲将本身携带的优势毒株传给子代 ,从病毒株本身来看子宫内感染以变异株为多。HBV在母儿间传播中变异逐渐积累形成两代间所携病毒群的差异。

This study consisted 6 female HBV carriers and 6 fetus who infected HBV in the womb.S gene 451～660 nucleotide,C gene 2022～2301 nucleotide were conducted by direct sequencing.The results showed that the homology of HBV sequence between mothers and fetus was very high.The mutations of 530、546、581 nucleotide in the S gene caused 126、131、143 amino acids substitution.In the C gene,variant virus strain and wild virus strain were detected among mother and her fetus.The data suggested that most mothers transmited the dominant virus strain to fetus,few mothers transmited the minor virus strain to fetus.