定心藤多糖提取工艺优化及其清除自由基研究

Study on Optimization of Extraction Process of Mappianthus iodoies Hand. MaZZ. Polysaccharide and Its Scavenging Effect on Radical

目的研究定心藤Mappianthusiodoies Hand.MaZZ.多糖的提取工艺及其清除自由基的能力。方法在单因素试验的基础上,通过正交试验确定水提取定心藤多糖的最佳条件;采用羟自由基和DPPH自由基体系,对定心藤多糖清除自由基的能力进行研究。结果水提取定心藤多糖的最优条件为:提取温度80℃,提取时间2 h,料液比1∶8。多糖浓度为1200μg/ml时,其对羟自由基的清除率达到70.65%;浓度为500μg/ml时,对DPPH自由基的清除率可达33.54%。结论得到了水提取定心藤多糖的最佳条件,探明了定心藤多糖对羟自由基和DPPH自由基的清除作用。

Objective To study the extraction process of Mappianthus iodoies Hand.MaZZ.Polysaccharide and Its Scavenging Effect on Radical.Methods Based on single factor test,the extraction conditions were optimized by orthogonal test.The scavenging capacity of Mappianthus iodoies Hand.MaZZ.Polysaccharide was investigated using.OH and DPPH free radical system.Results The optimal extraction conditions were as follows： extraction temperature of 80℃,liquid ratio of 1∶ 8,extraction duration of 2 h.As concentration of polysaccharide was 1200μg/ml,its scavenging rate on.OH reached 70.65%.As concentration of polysaccharide was 500μg/ml its scavenging rate on DPPH reached 33.54%.Conclusion The optimum extraction technology of Mappianthus iodoies Hand.MaZZ.polysaccharid with water extraction was obtained in this study,and it had strong scavenging effect on hydroxyl radical