HPLC法测定补脾益肠丸中苦杏仁苷含量

HPLC Determination of Amygdalin Content in Bupi Yichang Pill

目的：为了更好的控制补脾益肠丸质量,对补脾益肠丸中苦杏仁苷的含量进行测定。方法：采用Zorbax SB-C18柱（250 mm×4.6 mm,5μm）;流动相：乙腈-0.1%磷酸溶液（8∶92）;检测波长为210 nm;柱温35℃。结果：苦杏仁苷在10.25~512.5μg范围内线性关系良好,r=0.9999,平均回收率98.91%,RSD=0.48%。结论：此方法操作简便、准确、重现性好,可有效的控制补脾益肠丸的质量。

Objective： In order to better control quality of Bupi Yichang pill, amygdalin content in Bupi Yichang pill was measured. Method ： A Zorbax SB-C 18 column （ 250 mm x 4.6 mm, 5 μm ）; mobile phase ： acetonltrile -0.1% phosphoric acid （ 8：92 ）; detection wavelength was 210 nm; column temperature of 35 μC . Results ： The amygdalin linear in the range of 10.25- 512.5 μ g had good relationship （ r=0.9999 ）, the average recovery rate was 98.91% （ RSD=0.48% ）. Conclusion ： This method is simple, accurate with good reproducibility, and can effectively control the quality of Bupi Yichang pill.