DnaJ-like基因在不同环境胁迫下的表达研究

Expression analysis of DnaJ-like protein gene from bean under various environment stresses

Pv SR6( Phaseolus vulgaris stress- related)基因编码一种菜豆 Dna J- like蛋白。Northern blot结果表明 :Pv SR6基因在未处理的菜豆叶片中表达较少 ,重金属 ( Hg2 +和Cd2 + )、机械损伤、UV、高温和水杨酸等环境胁迫能强烈地促进其基因的转录 ,推测 Dna J- like蛋白在保护细胞膜和酶蛋白的结构和功能及提高植物的抗逆性方面有重要作用。

PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library.Northern blot analysis revealed that the transcripts of PvSR6 were at low levels in unstressed bean leaves,but the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid.These suggested that PvSR6 might have important roles in resistance to heavy metals and various environmental stresses by maintaining the structure and function of membranes and enzymes.