基因枪轰击谷子幼穗获得转基因植株

Production of transgenic millet plants via particle bombardment

以 JQ- 70 0型国产基因枪轰击豫谷 2号谷子幼穗 ,在 1 50 mg/L卡那霉素选择培养基上筛选到 90 8块抗性愈伤组织 ,其中 ,绿芽块愈伤 5块 ,共分化出 1 6株绿苗。组织化学检测GUS表达 ,获得 3株阳性植株 ,Southern杂交证明 1株为阳性。以轰击总外植体计算的转基因植株频率为 0 .0 5%。

Inflorescence of millet crop,Yugu No.2,was bombarded by JQ 700 particle gun.The plasmid of pBI121,which containing GUS reporter gene and npt Ⅱ selective marker gene,was used in this study.Totally 908 kanamycin resistance (Kan R) calli was produced in selective medium with 150 mg/L Kanamycin.Five of Kan R calli were characterized out with green shoots which differenciated 16 green plantlets.GUS histochemical assay demonstrated that three of them is positive.Southern blot confirmed that one plant is transgenic.The transgenic frequency is 0.05%.