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梅花鹿鹿茸双向电泳蛋白提取条件筛选 被引量：2

Optimization of Two-dimensional Electrophoresis of Sika Deer Antlers
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摘要 根据鹿茸组织血液含量较高、骨化较为严重的特点,设计了3种适合双向电泳的鹿茸蛋白质提取方法。筛选出2D-cleanup纯化为鹿茸蛋白质双向电泳的最佳提取方法。 We designed three experiments according to higher blood levels of deer antler tissue and more severe ossification,2D-clean up purified protein was best extraction method for two-dimensional electrophoresis of sika deer antlers.
作者 刘华淼 邢秀梅 杨福合
机构地区 江苏科技大学 中国农业科学院特产研究所吉林省省部共建特种经济动物分子生物学国家重点实验室
出处 《特产研究》 2013年第2期1-2,18,共3页 Special Wild Economic Animal and Plant Research
基金 中国农业科学院院长基金(201004)
关键词 鹿茸 蛋白质组 蛋白提取 velvet proteome protein extraction
分类号 Q503 [生物学—生物化学]
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参考文献7

二级参考文献54

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共引文献22

同被引文献19

  • 1文灿,余汇洋.Fibulin-5与肿瘤的研究进展[J].局解手术学杂志,2006,15(5):337-338. 被引量：2
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  • 3Zhang S, Wang S, Huang J, et al. High-specificity quantification method for almond-by-products, based on differential proteomic analysis[J]. Food Chemistry,2016, 194:522-528.
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  • 6Montowska M, Pospiech E. Differences in two- dimensional gel electrophoresis patterns of skeletal muscle myosin light chain isoforms between Bos taurus, Sus scrofa and selected poultry species[J]. Journal of the Science of Food & Agriculture,2011,91 (13):2449-2456.
  • 7Laville E, Sayd T, Morzel M. Proteome changes during meat aging in tough and tender beef suggest the importance of apoptosis and protein solubility for beef aging and tenderization[J]. Journal of Agricultural & Food Chemistry, 2009, 57(22): 10755-10764.
  • 8Montowska M, Pospiech E. Species-specific expression of various proteins in meat tissue: Proteomic analysis of raw and cooked meat and meat products made from beef, pork and selected poultry species[J]. Food Chemistry, 2013,136(3-4):1461-1469.
  • 9高亮,乔晓强,梁振,张丽华,霍玉书,张玉奎.基于在线二维色谱的不同生长时期鹿茸的比较蛋白质组分析[J].色谱,2010,28(2):146-151. 被引量：7
  • 10龙峰.TCA/丙酮沉淀对脑脊液标本中蛋白质双向电泳的影响分析[J].四川医学,2010,31(4):532-533. 被引量：7

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特产研究
2013年 第2期

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