AT1R基因多态性与煤矿井下工人原发性高血压的关系

Correlation of polymorphism of AT1R gene with essential hypertension in coal miners

目的分析血管紧张素Ⅱ 1型受体(AT1R)基因多态性与原发性高血压(EH)的关系。方法选择煤矿井下接尘工人412人为调查对象(病例组与对照组各206人),进行职业流行病学调查,并收集调查对象空腹静脉血5ml,酚氯仿法提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测AT1R基因多态性。比较两组间的基因型、等位基因频率的分布差异,分析基因型、等位基因频率与EH的关系。结果AT1R基因多态性AA、AC、CC基因型在病例组和对照组中的分布分别为92.23%、7.77%、0.00%和86.41%、13.10%、0.49%,两组比较,差异无统计学意义(P>0.05)。等位基因A、C在病例组和对照组中分别占96.12%、3.88%和92.96%、7.04%,差异无统计学意义(P>0.05)。结论该试验未发现AT1R基因多态性与煤矿井下作业人员EH有关。

Objective To analyze the correlation of polymorphism of AT1R gene with essential hypertension. Methods 412 underground pit dust exposed coal miners were taken as the observed subjects and equally divided into case group and control group. Population--based case--control study was conducted to explore the correlation of polymorphism of AT1R gene with essential hypertension in coal miners using PCR --RFLP techniques. Through comparison of the differences of genotype and allele frequency distribution between two groups, the correlation of genotype and allele frequencies with essential hypertension was analyzed. Results The AT1R gene polymorphisms AA, AC, CC genotype in both groups were 92.23%, 7.77%, 0. 00% and 86.41%, 13.10%, 0.49% respectively. The difference was not statistically significant （P〉0.05）. Allele A and C in both groups accounted for 96.12%, 3.88% and 92.96%, 7.04% respectively. The difference was not statistically significant （P〉0.05）. Conclusions The correlation of AT1R gene polymorphism with hypertension in coal miners is not found in this experiment.