HSP1A1基因+190G>C多态与有氧运动能力表型指标的关联性研究

Relationship of HSPA1A +190G>C Genetic Polymorphisms with the Phenotypic indicators of Aerobic Capacity

目的：探讨热休克蛋白70（Heat Shock Protein 70,HSP70）基因（HSPA1A）＋190G〉C单核苷酸多态性（Single Nucleotide Polymorphism,SNP）与有氧运动能力表型指标的关系。方法：试验对象为400名大学一年级学生,其中男生300名,女生100名,均为汉族。受试者以95%~105%个体通气无氧阈（VT）进行每周3次,每次5 000 m,为期18天的长跑训练。测定训练前后摄氧量绝对值（VO2）、摄氧量相对值（rVO2）、通气无氧阈（VT）和每分钟通气量（VE）,并进行跑节省化试验。采用ABI7900HT的Taqman基因分型技术,检测HSP1A1基因＋190G〉C位点基因型。结果：HSP1A1＋190G〉C位点基因多态性与有氧运动能力表型存在关联,随着G等位基因的增加,个体有氧运动能力也在增加;有氧耐力训练前后,不同基因型者VO2max、rVO2max、VT/VO2和VE均无统计学差异（P〉0.05）;有氧耐力训练前GG基因型个体RE/HR值显著低于GC和CC基因型者,且随着C基因型的增加,RE/HR值也逐渐增加,趋势检验发现具有剂量效应关系（Ptrend〈0.05）;有氧耐力训练后不同基因型RE/HR和RE/VE值以及他们的变化率间差异具有统计学意义（P〈0.05）,且随着C基因型的增加,RE/HR和RE/VE值以及他们的变化率也逐渐增加,趋势检验发现具有剂量效应关系（Ptrend〈0.05）。结论：HSPA1A基因＋190G〉C位点与有氧耐力训练后有氧运动能力表型存在关联,GG基因型个体具有较高的有氧运动能力水平。

Objective：To explore the associations between the heat shock protein 70 gene（HSPA1A）＋190G＆gt;C polymorphisms and the aerobic capacity to aerobic endurance training program among healthy freshmen.Methods：A total of 400 healthy freshmen were included in the present study,including 300 boys and 100 girls and all were Han nationality.All subjects performed an aerobic endurance training program of 18-week 5000m running,3 times a week with the intensity of 95%-105% aerobic threshold.VO2max,rVO2max,VE,VT and VE were measured before and after the aerobic endurance training.Polymorphisms of HSP1A1 ＋190G＆gt;C were detected by Taqman single nucleotide polymorphism（SNP）allelic discrimination by means of an ABI7900HT.Results：The ＋190G＆gt;C SNP in HSPA1A gene was associated with aerobic capacity in men and women,individual aerobic capacity is also increasing with the increase of the G allele.People with the HSPA1A GG genotype have lower RE/HR values,than carriers of the HSPA1A C190 allele after the endurance training（P＆lt;0.05）,and there was a dose response relationship among the three genotype groups（Ptrend＆lt;0.05）.After the endurance training the RE/HR and RE/VE values were significant reduced,and these values in GG genotype were significant lower than the C allele,and there were also dose response relationships among the three genotype groups（Ptrend＆lt;0.05）.Conclusion：The ＋190G＆gt;C SNP in HSPA1A gene was not only associated with aerobic capacity but also with endurance training promotion.