灵芝胞外多糖的分离纯化及生物活性

ISOLATION, PURIFICATION AND BIOACTIVITIES OF EXOPOLY SACCHARIDES FROM FERMENTED BROTH OF GANODERMA LUCIDUM

目前,灵芝多糖的抗肿瘤及免疫活性已得到人们的广泛关注[1]。灵芝子实体多糖和发酵过程中产生的胞内和胞外多糖已被国内外的研究者证实都是有效多糖[2]。因此利用深层发酵来大规模地生产生物活性多糖是目前灵芝开发利用技术的热点。国内对灵芝培养基的组成、培养方法等已?..

The exopolysaccharides of \%Ganoderma lucidum\%(GLEP) extracted from the fermentation broth after removing protein by Sevage and protease digestion procedures, were applied to a column of DEAE\|cellulose(OH\+- form), and eluted stepwise with distilled water, sodium hydrogen carbonate (0.1 mol/L, 0.3mol/L,0.5mol/L successively) and 0 1mol/L sodium hydroxide. Five fractions were obtained, and the main fraction was known as GLEP\|Ⅰ, furthermore subjected to chromatography on a column of SepharoseC1\|6B, eluted at a flow rate of 30mL/(cm\+2·h), the relative viscosity of sample solution of 1.5. Two fractions, GLEP IFr1 and GLEP IFr2 with a ratio of 3.8∶1, were obtained,. Molecular weight of GLEP IFr1 and GLEP IFr2 was estimated to be \{38 000\} and \{22 000\} Dalton respectively by Membrane Osmometer. The animal test showed that GLEP IFr1 could inhibited the growth of Sarcoma 180 tumor in mice. The average inhibition ratio was 57.4%(i.p.10mg/kg for 10 days). The result of immunological activity showed that GLEP IFr1 could significantly improve macrophage cytophagy.