微孔过滤法测定红细胞变形性的统一理论模型

A UNIFIED THEORETICAL MODEL OF MICROPORE FILTRATION FOR ASSESSING THE RED BLOOD CELL DEFORMABILITY

在红细胞悬浮液过滤前后压积不变的合理假设下 ,并考虑到微孔滤膜堵塞现象 ,建立了微孔过滤法测定红细胞变形性的统一理论模型—流导模型。该模型利用滤膜相对流导Cr 与悬浮液滤过体积V之间的线性关系表达实验结果。利用这一模型 ,从微孔过滤实验得到了分别反映平均变形性和堵塞信息的两个微观参数 :红细胞平均相对过孔阻力 β 和导致滤膜堵塞的红细胞比例ε。流导模型对各种不同的过滤方法给予统一的描述 ,对现有的过滤方法或可能出现的新方法 ,只要过滤是连续的 ,从而跨膜压差和流量的变化是连续的 ,流导模型的控制方程以及由此得出的红细胞变形性参数 β 和ε的表达式均适用。

A unified theoretical model of micropore filtration for assessing the red blood cell (RBC) deformability is developed, in which the hematocrit in the suspension is assumed to be constant across the filter and the filter clogging phenomenon is taken into consideration. With this model, the experimental data are expressed in terms of a linear relationship between the flow conductance C and the filtered volume V of suspension, from which two microscopic parameters are determined, i.e. the relative resistance β of RBC's passage of the pore with respect to the buffer and the proportion ε of RBCs leading to the filter clogging. Although different experimental methods usually produce different type of curves, this model is able to provide a unified description of them. The relationship of C versus V as well as the expressions for β and ε derived in this model are valid for any experimental methods (currently available or to be developed), if only the filtration is continuous so that the filtration pressure and flow rate vary continuously.