定量研究脑缺血及再灌注下微血管的通透性

THE QUANTITATIVE STUDY ON MICROVASCULARPERMEABILITY UNDER POST-ISCHEMIC REPERFUSION

利用示踪剂FLNa在脑缺血及再灌注的动物模型上 ,通过活体观察和测定血液、脑等脏器的荧光强度 ,以及对软脑膜微血管荧光图象的平滑处理与定量分析,研究软脑膜微血管的通透性 ,探讨脑缺血及再灌注对微血管通透性的影响及内在规律。实验结果表明 :缺血、缺血及再灌注会引起微血管内皮细胞的损伤 ,导致微血管通透性增大 ,这种损伤一般发生在缺血或再灌注早期 ,虽然各脏器微血管都受到损伤 ,但其荧光值不同 ,说明各脏器抗缺血与缺氧的能力不同 ,其中脑与脾最强 ,肝、肾最弱 ,而肺的测量值比较特殊 ,这一特殊性与肺的自身结构有关 ;长时间的血液再灌注对损伤有修复作用。此外 ,还提出了图象处理与分析的方法 ,建立了荧光物质在微血管外的扩散方程 ,并求出了扩散速度。

The rat model of cerebral ischemia with reperfusion was employed to observe intravital microvessels and to measure the fluorescence intensities of blood, brain and other five internal organs of the body for studying the permeability of cerebral microvessels with fluorescent tracer FLNa. It was designed to insure that the image of cerebral microvessels was smoothly processed and was quantitatively analyzed in order to find the effect and inherent law of ischemia with reperfusion on the permeability of cerebral microvessels. Experiment results show that ischemia and ischemia with reperfusion do harm on endothelial cells of the capillary;In this case the permeability was obviously enhanced as compared with that in normal condition, and the long duration of reperfusion is helpful to trauma repairing. Under the same conditions, their different fluorescence intensities show that their resistance to ischemia and lacking of oxygen isn't identical, and the degree of harm varies considerably in internal organs, where the most serious is the endothelial cells of liver and kidney, the lesser those of brain and spleen. The condition of lung is an exception and the speciality of lung is related to its structure. Besides, the method of image process and quantitative analysis was established to find diffuse equation and diffuse speed of fluorescence agent after passing through the wall of microvessels.