一种新型的α-纤维蛋白原酶对血小板聚集的影响

Effect on a Novel α-Fibrinogenase for Platelet Aggregation

SDS-聚丙烯酰胺凝胶电泳 ( SDS- PAGE)检测中国眼镜蛇毒蛋白酶水解纤维蛋白原的作用方式 ,发现该蛋白酶可迅速水解纤维蛋白原 Aα链 ,对 γ链作用较弱 ,对 Bβ链无作用 ,是一种新型的α-纤维蛋白原酶 .用比浊法测定血小板聚集率 ,证实中国眼镜蛇毒蛋白酶低剂量 ( 0 .0 2 5mg/kg)、中剂量 ( 0 .0 5mg/kg)以及高剂量 ( 0 .1 mg/kg)体内给药浓度依赖性地抑制 ADP( 1 0 μmol/L)和胶原 ( 1 0 0 mg/L)

As revealed by SDS polyacrylamide gel electrophoresis (SDS PAGE),a Chinese cobra venom proteinase preferentially cleaved Aα andsubsequently γ chain of fibrinogen, but left the Bβ chain unaffected,suggesting that it was a novel α fibrinogenase Rabbit platelet aggregation was measured turbimetrically When tested in vivo at the dosages of 0 025 mg/kg, 0 05 mg/kg and 0 1 mg/kg, this Chinese cobra venom proteinase inhibited rabbit platelet aggregation induced by 10 μmol/L of ADP or 100 mg/L of collagen in a dose dependent manner