试管牛和试管羊胚胎性别的鉴定

Sexing the IVF Bovine and Goat Embryos

奶牛和山羊的卵母细胞经过体外成熟、体外受精和体外发育至桑椹期 ,在显微操作下 ,吸取4～6个胚胎细胞 ,应用巢式PCR技术对其DNA进行SRY基因的测定以进行胚胎性别鉴定。共有27枚转有外源基因的试管牛胚胎和207枚转基因试管羊胚胎进行了SRY基因检测。选取10枚经过性别鉴定的牛胚胎移植于8头受体母牛和124枚已知性别的羊胚胎移植于44头受体母羊 ,移植后妊娠率分别为37 5% (牛 ,3/8)和61 4% (羊 ,27/44)。最后产生1头分娩牛犊和2头流产胎牛以及14头分娩羊羔和2头流产胎羊 ,它们的性别与胚胎性别鉴定的结果完全相符。

The oocytes of bovines and goats were subjected to in vitro maturation,in vitro fertilization(IVF)and in vitro development upto morula stage 4～6 embryonic cells were aspirated with micro manipulating and the embryo sexes were identified by detecting SRY gene with nested PCR procedure A total of 27 transgenic IVF bovine and 207 transgenic IVF goat embryos were performed SRY gene detection 10 bovine and 124 goat sexed embryos were selected to be transferred into 8 bovine and 44 goat recipients,respectively,leading to the pregnant rates of 37 5%( in bovine,3/8)and 61 4%(in goat,27/44) In the end,1 cattle and 14 goatlets were born at term but 2 fetal bovine and 2 fetal goats were miscarried Their sexes were fully in accordance with the embryonic sex predetermination