二十五味珊瑚胶囊对退变颈椎间盘组织磷脂酶A2的影响

Effects of Jinzhuyalong Ershiwuwei coral capsule on PLA2 in degenarated cervical intervertebral discs

目的观察二十五味珊瑚胶囊对模型动物退变颈椎间盘组织磷脂酶A2（PLA2）活性的影响，探讨二十五味珊瑚胶囊治疗颈椎病的作用机理。方法SD大鼠（SPF）60只，随机抽签分为对照组（15只）、模型对照组（15只）、模型服药1组（15只），模型服药2组（15只）；模型服药1、2组分别给予二十五味珊瑚胶囊粉剂0．33×5g／kg、0．66×5g／kg，加蒸馏水1ml稀释灌胃给药，1次/d，持续90d。选用动力失衡性颈椎病动物模型，90d后取c4～5颈椎间盘，采用催化活性与微孔比色相结合的方法测定PLA2活性。结果模型组颈椎间盘PLA2活性（12．37±4．21）IU／mg·min^-1，明显高于对照组（2．56±1．19）IU／mg·min^-1（P〈0．05）。模型服药1组、模型服药2组颈椎间盘PLA2活性分别为（8．56±2．13）IU／mg·min-1、（5．58±2．39）IU／mg·min^-1，均低于模型对照组（12．37±4．21）IU／mg·min^-1护均〈0．01）。结论二十五味珊瑚胶囊可下调退变颈椎间盘中PLA2的活性，可减少多种炎症介质的合成。

Objective To study the anti- inflammation mechanism of Jinzhuyalong Ershiwuwei coral capsule on cervical spondylosis by investigating Jinzhuyalong Ershiwuwei coral capsule on PLA2 in degenerated cervical intervertebral discs. Methods 60SD rats （SPF） were divided into a control group （15）, a medicine model control group （15）, a medicine modle 1 group （15）, and a medicine model 2 group （15） by random lottery; Medicine model 1,2 groups were given Jinzhuyalong Ershiwuwei coral capsule powder 0.33 X 5 g/kg 0.66×5 g/kg, and added 1 ml distilled water dilution lavage, I/d, 90 d, The unbalance of power cervical spondylosis animal models were chosen and C4~5 neck intervertebral discs were taken after 90 days. Millipore colorimetry methods were used to measure PLA2 activity in C4~5 cervical intervertebral discs. Results The PLA2 activity of the cervical intervertebral disc （12.37±4.21） lU/mg·min-1 in the model group was significantly higher than the control group （2.56±1.19）IU/mg·min-1 （P〈0.05）. The cervical intervertebral disc PLA2 activity of medicine model group （8.56±2.13）IU/mg·min-1 and medicine model group 2 （5.58±2.39）IU/mg·min-1 was lower than the model control group（12.37±4.21）IU/mg·min-1, with significant differences （both P〈0.01）. Conclusion Jinzhuyalong Ershiwuwei coral capsule could down regulate PLA2 activity in degenerated cervical intervertebral discs, and also decrease the production of some inflammaroty mediators.