植物叶片蛋白质双向电泳技术的改进与优化

Improvement in the Two dimensional Electrophoresis of Proteins from the Leaves of Plant

通过对传统的双向电泳方法进行改进与优化，得到了一种适合于分析植物叶片蛋白质的双向电泳新方法。用改进后的方法对水稻不同时期成熟叶片蛋白质进行双向电泳分离，结果显示其稳定性和重复性好，分辨率较高,经考马斯亮蓝染色后可分辨出300多个蛋白质（肽）点。它们的等电点和分子量主要分布于pI4.1-8.2和10-100kDa之间。本文还就实验过程中出现的一些技术问题进行了讨论。

Based on the method of O'Farrell,an improved procedure for the two dimensional gel electrophoresis of leaf proteins is presented.The two dimensional separation of proteins from the mature leaves of different developmental stages of rice provides distinct and steady results.The detected protein(peptide)spots stained by Coomassie brilliant blue R 250 are more than 300,with isoelectric points(pI)ranging from 4 1 to 8 2 and molecular weights(MW)from 10 kDa to 100 kDa.