结核分枝杆菌与两种人工关节介面黏附特点对比研究

Adherence Condition of Mycobacterium Tuberculosis on 2 Kinds of Artificial Joint Interface

目的探讨结核分枝杆菌黏附性。方法将表面多孔涂层和羟基磷灰石涂层实验材料每组各取20片,将每片实验材料在无菌条件下悬吊于M960培养基中,与结核分枝杆菌共同培养。每个M960培养基培养28 d。用721分光光度计(波长590 nm、光镜1.0 cm、蒸馏水调零)测定培养基菌液的光密度(OD)值。用扫描电镜观察结核分枝杆菌与两种介面的黏附情况。结果两种材料的OD值无显著性差异(P>0.05)。结核分枝杆菌在多孔介面黏附量高于羟基磷灰石涂层介面(P<0.05)。结论在为关节结核患者行人工关节置换时,选择羟基磷灰石涂层比多孔涂层更有优势。

Objective To compare the adherence condition of mycobacterium tuberculosis on 2 kinds of artificial joint intertace. Methods The Chromium-Cobalt-Alloy with hydroxyapatite ceramic coating and porous coating were cocultured with the bacterial liquid of myco- bacterium tuberculosis. The optical density of mycobacterium tuberculosis in different interfaces were detected by turbidimetry. Adherence conditions were observed by electron microscope scanning. Results There was no significant difference between two groups in the optical density value （P〉0.05）. The electron microscope scanning showed that more mycobacterium tuberculosis adhered to the porous coating than hydroxyapatite ceramic coating （P〈0.05）. Conclusion The hydroxyapatite ceramic coating has more advantages to the porous coating in prosthetic replacement for patients with joint tuberculosis.