塞来昔布对神经胶质瘤细胞U251增殖及凋亡的影响

Effect of Celecoxib on Proliferation and Apoptosis of U251 Glioma Cells

目的探讨非甾体类抗炎药(NSAIDs)对神经胶质瘤细胞增殖的影响。方法选用神经胶质瘤细胞U251,分别加入0μmol/L、10μmol/L、20μmol/L、40μmol/L和80μmol/L的塞来昔布进行处理。四甲基偶氮唑盐比色(MTT)法检测U251细胞增殖水平及抑制率,流式细胞术检测细胞凋亡率。结果随塞来昔布浓度的增加,神经胶质瘤细胞U251增殖水平明显降低,增值抑制率升高。不同浓度和不同作用时间,塞来昔布对U251细胞增殖抑制率有显著性差异(P<0.05);流式细胞术细胞凋亡率检测显示,80μmol/L塞来昔布处理48 h的U251细胞凋亡率(17.86%)较0μmol/L(11.23%)增高(P<0.05)。结论塞来昔布可抑制人胶质瘤细胞U251的生长和增殖,促进U251的凋亡发生,以80μmol/L为佳。

Objective To explore the effect of celecoxib on proliferation and apoptosis of the glioma cells. Methods The glioma cell U251 was used and disposed with different densities of celecoxib （0 μmol/L, 10μmol/L, 20 μmol/L, 40 μmol/L and 80 μmol/L） for 24 h, 48 h and 72 h. The methyl thiazolyl tetrazolium （MTT） assay was used to detect the tumor cell proliferation and flow cytometry was used to detect the tumor cell apoptosis rate. Results The Glioma U251 cells proliferation were significantly decreased with the increase of density of celecoxib in vitro, and there was significant difference in the inhibited rate in different density and different time （P〈0.05）. The apoptosis rate was higher in the density of 80μmol/L （17.86%） than in that of 0 μmol/L （11.23%） （P〈0.05）. Conclusion Celecoxib can inhabit the pro- liferation of glioma U251 cells, and promote the apoptosis especially with the density of 80 μmol/L.