透析培养

Dialysis culture

本文讨论了有效利用透析技术从发酵液中及时转移低分子杂质混合物 ,从而获得高密度发酵细胞的方法。文章从反应系统、工艺策略、膜相关性能、应用实例、生产性放大等方面说明了利用透析技术以达到高浓度细胞发酵的有效性和可靠性。透析技术不仅克服了微孔过滤和超滤中存在的膜孔堵塞弊端 ,而且如果应用“营养分离”补料策略 ,还可以防止营养物质的损失而使培养基被高效利用。在实验室条件下 ,透析培养的潜力通过两种反应模型进行演示 :内置式透析反应器和带外部透析环路的反应器。在不同微生物 (葡萄球菌 ,大肠杆菌 ,极端环境型微生物 ,乳酸杆菌 )的透析培养中 ,细胞浓度都比其它发酵方法提高了 30倍。因此采用这种方法 ,不需进行费时的培养基优化工作即可实现高浓度的细胞发酵。对动物细胞培养而言 。

Dialysis techniques are discussed as a means for effective removal of low-molecular-mass components from fermentation broth to reach high cell density. Reactor systems and process strategies, the relevant properties of membranes and examples for high-density fermemtation with dialysis, and problems related to scale-up are addressed. The dialysis technique has turned out to be very efficient and reliable for obtaining high cell densities. By applying a 'nutrient-split' feeding strategy, the loss of nutrients can be avoided and the medium is used very efficiently. The potential of dialysis cultures is demonstrated on the laboratory scale in a membrane dialysis reactor with an integrated membrane and in reactor systems with an external dialysis loop. In dialysis cultures with different microorganiams the cell densities achieved were up to 30 times higher than those of other fermentation methods. The technique enable high cell densities to be attained without time-consuming medium optimization. For animal cell cultures the concept of a fixed bed coupled with dialysis proved to be very effective.