ADAM33基因遗传多态性与支气管哮喘易感性关系的Meta分析

Association between ADAM33 genetic polymorphsim and susceptibility of asthma:A Meta-analysis

目的:综合评价解整合素金属蛋白酶33(ADAM33)基因Met764Thr和Pro774Ser位点多态性与哮喘易感性的关系。方法:按照统一的检索策略,检索Pubmed、Ovid-Medline、CNKI及维普数据库中有关ADAM33基因多态性与哮喘易感性关系的病例-对照研究,按照纳入和排除标准选择文献提取相关信息,应用Review Manager 5.0软件进行Meta分析。结果:本研究纳入国内外14篇合格文献,其中Met764Thr位点12篇,共3 418例哮喘病例和3 520例对照;Pro774Ser位点8篇,共2 793例哮喘病例和3 207例对照。Meta分析结果显示,携带764位点Met/Thr或Thr/Thr突变基因型患哮喘的危险性是野生型的1.56倍(OR=1.56,95%CI=1.09～2.22),Pro774Ser位点突变基因型也与哮喘危险性升高有关(OR=1.39,95%CI=1.00～1.93)。将人群分层后,该两个位点多态性与哮喘的关联均仅见于中国人群(764位点:OR=2.73,95%CI=1.79～4.17,774位点:OR=2.32,95%CI=1.30～4.15)。结论:ADAM33基因764和774位点的突变与哮喘的易感性升高有关。

Objective： To explore whether the polymorphisms of Met764Thr and Pro774Ser in ADAM33 （A Disintegrin and Metalloproteinase 33） gene contribute to the susceptibility to asthma. Methods：According to the same criteria, all the clinical studies related to the polymorphisms of ADAM33 gene and asthma were searched through PubMed, Ovid-Medline, CNKI, Weipu database. All retrieved articles were screened and evaluated followed by Meta-analysis using RevMan 5 software. Results：Fourteen studies were in- cluded based on the selection criteria. In these studies, twelve of them investigated the codon 764 of ADAM33 （3 520 controls and 3 418 asthmatic patients） and eight studied the codon 774 （3 207 controls and 2 793 asthmatic patients）. The results of Meta-analys is showed the genotype of 764Met/Thr and 764Thr/Thr have higher susceptibility（ 1.56 fold）to be asthmatics than the one with the geno- type of 764 Met/Met （ OR = 1.56, 95% CI = 1.09-2.22）. The mutant genotype of ADAM33 codon 774 was also associated with in- creased asthma risk（ OR = 1.39, 95% CI = 1.00-1.93 ）. Furthermore, subgroup analysis indicated that the association between these two loci and asthma could only be found in Chinese population （ codon 764 ： OR = 2.73, 95% CI = 1. 79-4.17 ; codon 774 ： OR = 2.32, 95% C1 = 1.30-4.15 ）. Conclusion ： Polymorphisms of ADAM33 codons 764 and 774 seem to be involved in elevated risk of asthma.