正交试验优选清肾颗粒醇提工艺

Optimization of Ethanol Extraction Technology of Qingshen Granule with Orthogonal Test

目的优选清肾颗粒的最佳醇提工艺。方法采用正交试验法,以大黄素、大黄酚、大黄素甲醚3种成分总含量及丹参酮ⅡA含量、干浸膏得率为考察指标,优选最佳醇提工艺。结果清肾颗粒最佳醇提工艺为:醇浓度为80%,加醇量8倍,提取时间2 h,提取次数为3次。结论该提取工艺设计合理,简单易行。

Objective To establish the optimal ethanol extraction technology of Qingshen Granule.Methods The total content of emodin,chrysophanol and physcion,the content of tanshinoneⅡA and dry extact rate was set as indexes,orthogonal test was adopted to optimize the technology.Results The optimum extraction conditions were as follows：8 times of 80% alcohol,refluxing 3 times and 2 hours for each time.Conclusion The optimum technology of Qingshen Granule is simple,stable and effective.