RAD51_(G135C)单核苷酸多态性及其蛋白表达在非小细胞肺癌中的意义

The clinical significance of RAD51_(G135C) single nucleotide polymorphisms and protein expression in non-small cell lung cancer

目的探讨RAD51G135C单核苷酸多态性及RAD51蛋白过表达与非小细胞肺癌(NSCLC)发病风险、临床病理特征的相关性。方法选择80例NSCLC患者为病例组,40例非肿瘤肺疾病患者为对照组。以PCR-RFLP技术,检测病例组和对照组的RAD51G135C基因型,比较不同基因型与NSCLC危险性及其临床特征的关系;采用免疫组化法检测RAD51蛋白表达情况,统计分析RAD51蛋白表达与NSCLC危险性及临床病理特征的关系。结果 (1)RAD51G135C基因型G/G、G/C和C/C在病例组的分布频率分别为77.5%、15.0%和7.5%,在对照组的分布频率分别为92.5%、7.5%和0.0%;与携带G/G的个体相比,携带RAD51G135C变异基因型(G/C和C/C)的个体具有更高的患癌风险(P<0.05)。(2)病例组RAD51蛋白过表达率为31.25%(25/80),高于对照组的2.5%(1/40)(P<0.05)。(3)RAD51G135C单核苷酸多态性与RAD51蛋白过表达密切相关(r=0.347,P<0.05);但两者均与NSCLC临床病理参数无关(P>0.05)。结论 (1)RAD51G135C基因多态性、RAD51蛋白过表达与NSCLC发病风险有关,携带RAD51变异基因型和(或)存在RAD51蛋白过表达的个体易患肺癌;(2)RAD51G135C基因多态性可能与RAD51蛋白过表达密切相关。

Objective To investigate the RAD51G135C single nucleotide polymorphisms and RAD51 protein expression in non-small-cell lung carcinoma（NSCLC）.Methods A total of 80 NSCLC cases and 40 controls with pulmonary non-neoplastic diseases were collected.The genotypes of RAD51G135C and the expression of RAD51 protein were analyzed by PCR-RFLP and immuonohistochemisty,respectively,for correlation analysis.Results The genotype frequencies of G/G,G/C and C/C were 83.7%,15.0% and 7.5%,respectively,in NSCLC patietns;and 92.5%,7.5% and 0.0%,respectively,in controls.When G/G served as the reference,there was significant increase risk of NSCLC of variant genotype（G/C and C/C,P0.05）.The RAD51 over-expression incidence was significant higher（31.25%,25/80） in NSCLC patients that in controls（2.5%,1/40;P0.05）.Although the genotype of RAD51G135C was significantly correlated with the over-expression of RAD51,there was no significant correlation between either of them and the clinicopathologic features of NSCLC（P 0.05）.Conclusion RAD51G135C single nucleotide polymorphisms and over expression of RAD51 protein,which are closely correlated,are risk factors of NSCLC.