摘要
3种真菌诱导子 (大丽花轮枝孢 (VerticilliumdahliaeKleb .)、葡枝根霉 (Rhizopusstolonifer (Ehrenb .exFr.)Vuill)和束状刺盘孢 (Colletotrichumdematium (Pers .)Grove) )处理青蒿 (ArtemisiaannuaL .)的发根 ,均能促进发根中青蒿素的积累 ,其中以大丽花轮枝孢的诱导效果最好 ;对细胞生长均没有明显影响。经大丽花轮枝孢处理的发根中青蒿素含量达 1.12mg/gDW ,比对照 (0 .77mg/gDW )提高 45 %。诱导子的作用效果与诱导子浓度、诱导子作用时间及发根的生长状态有关。对大丽花轮枝孢来说 ,诱导子作用的最适浓度为每毫升培养基含糖 0 .4mg ;发根在指数生长末期对诱导作用最敏感 ;在加入诱导子 4d后收获发根 ,发根中的青蒿素含量最高。
The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex Fr.) Vuill and Colletotrichum dematium (Pers.) Grove). Among these three elicitors, V. dahliae had the highest inducing efficiency, but none of them manifests any noticeable effects on the cell growth of the hairy root cultures. The artemisinin content of the hairy root cultures treated with V. dahliae elicitor was 1.12 mg/g DW, which was 45% higher than the control (0.77 mg/g DW). The results showed that elicitation was dependent on the elicitor concentration, the incubation period and the physiological stage at which the hairy root cultures were treated. In addition, the authors found that for V. dahliae , the optimum concentration was 0.4 mg carbohydrate per millilitre medium, the strongest response of A. annua hairy root cultures to the elicitation was at the late exponential growth stage, and the highest artemisinin content of the hairy root cultures was on the 4th day post treatment.
基金
TheNinthFive_YearPlanoftheNationalScienceandTechnologyKeyProjectofChina ( 96_C0 2_0 3_0 2 )
PriorityResearchFoundationoftheCh
