双探针原位杂交揭示稻属BB、CC和EE基因组之间的分化(英文)

Detection of Differentiation Among BB, CC and EE Genomes in the Genus Oryza by Two-probe Genomic in situ Hybridization (GISH)

利用双探针原位杂交技术 ,揭示了稻属Oryzaofficinalis复合体中BB、CC和EE基因组之间的分化。以标记的BB基因组 (来自二倍体的O .punctataKotechyexSteud .)的总DNA为探针 ,同BBCC基因组 (O .minutaJ.S .Presl.etC .B .Presel)的中期染色体杂交。结果表明 ,BB基因组的DNA探针同与四倍体O .minuta中的BB基因组的染色体之间有强烈的杂交信号 ,而与CC基因组的染色体之间的杂交信号很弱。而EE基因组 (来自O .aus traliensisDomin)的总DNA标记的探针同O .minuta基因组的所有染色体均有不同程度的杂交 ,且杂交信号的大小和强弱在BB基因组的染色体和CC基因组的染色体之间无显著的差异。由此证明 :(1)CC和BB基因组之间的分化程度很大 ;(2 )EE基因组同CC和BB基因组具有一定的亲缘关系 ;(3)CC基因组与EE基因组之间的关系较近而同BB基因组关系较远。

The genus Oryza consists of two cultivated species (O. sativa L. and O. glaberrima Steud.) and approximately 20 wild relative species widely distributed in the pan-tropics. These species have been classified into four complexes following the Vaughan's taxonomic system([1]). The O. officinalis complex is the largest complex in the genus, which includes ten species, having BE, CC, on, and EE genomes in the diploids as well as BBCC and CCDD genomes in the tetraploids. The relationships among the BE, CC, and EE genomes still remain unclear, although previous studies have indicated certain affinities of these genomes([2-4]). Genomic in situ hybridization (GISH) is a powerful technique to detect the relationships among the related genomes at chromosome and DNA levels. The objective of the present study was to investigate the relationships among the BE, CC and EE genomes in the genus Oryza by the two-probe GISH.